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Among those present when the sample as cut from the shroud were Cardinal Anastasio Ballestrero (Archbishop of Turin), Professor L. The laboratories were not told which container held the shroud sample.

Gonella (Department of Physics, Turin Polytechnic and the Archbishop's scientific adviser), two textile experts (Professor F. Because the distinctive three-to-one herringbone twill weave of the shroud could not be matched in the controls, however, it was possible for a laboratory to identify the shroud sample.

One-third received no further treatment, one-third was submitted to a weak treatment with 0.5% HCL (room temperature), 0.25% Na OH (room temperature) and again in acid, with rinsing in between.

The final third was given a strong treatment, using the same procedure except that hot (80° C) 5% HCL and 2.5% Na OH were used.

On the basis of the stylistic details and the historical evidence the cope could be dated at ~ AD 1290 - 1310 (reign of King Phillipe IV).

Because it was not known to what degree dirt, smoke or other contaminants might affect the linen samples, all three laboratories subdivided the samples, and subjected the pieces to several different mechanical and chemical cleaning procedures.

The second pair of subsamples was treated with a commercial detergent (1.5% SDS), distilled water, 0.1% HCL and another detergent (1.5% triton X-100); they were then submitted to a Soxhlet extraction with ethanol for 60 min and washed with distilled water at 70° C in an ultrasonic bath (method b).

The Oxford group divided the precleaned sample into three.

The Oxford group cleaned the samples using a vacuum pipette, followed by cleaning in petroleum ether (40° C for 1 h) to remove lipids and candlewax, for example.

Even for the first investigation, there was a possibility of using radiocarbon dating to determine the age of the linen from which the shroud was woven.

The size of the sample then required, however, was ~500cm, which would clearly have resulted in an unacceptable amount of damage, and it was not until the development in the 1970s of small gas-counters and accelerator-mass-spectrometry techniques (AMS), requiring samples of only a few square centimetres, that radiocarbon dating of the shroud became a real possibility. The shroud was separated from the backing cloth along its bottom left-hand edge and a strip (~10 mm x 70 mm) was cut from just above the place where a sample was previously removed in 1973 for examination.

Following this intercomparison, a meeting was held in Turin in September-October 1986 at which seven radiocarbon laboratories (five AMS and two small gas-counter) recommended a protocol for dating the shroud. The samples were then taken to the adjacent Sala Capitolare where they were wrapped in aluminium foil and subsequently sealed inside numbered stainless-steel containers by the Archbishop of Turin and Dr Tite.

In October 1987, the offers from three AMS laboratories (Arizona, Oxford and Zurich) were selected by the Archbishop of Turin, Pontifical Custodian of the shroud, acting on instructions from the Holy See, owner of the shroud. Samples weighing 50 mg from two of the three controls were similarly packaged.

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